Regulatory

Part:BBa_K1087004:Experience

Designed by: Tong Xu   Group: iGEM13_SCU_China   (2013-09-12)


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Applications of BBa_K1087004

User Reviews

UNIQ4245e95c28002feb-partinfo-00000000-QINU UNIQ4245e95c28002feb-partinfo-00000001-QINU http://2013.igem.org/Team:SCU_China/Project#Results

We assemblied BBa_K1087004 with mRFP to create BBa_K1087015, and we ligate BBa_K1087015 with RiboKey BBa_K145215 to create BBa_K1087021. Then, we transformed BBa_K1087015, BBa_K1087021 into E.coli DH5α,respectively. And we use Ptet+mRFP1(BBa_I13521) as positive control, irrelevant BBa_J61046 with no fluorescence gene as negative control. All of the constructs were expressed in high copy plasmid PSB1X3.

According to Data,the ribolock BBa_K1087004 works well, and its fluorescence intensity was only 2.6531% compared to positive control even at 19h, while that of lock & key (BBa_K1087021) was 91.7138% at 19h. The results suggest the key BBa_K145215 can hugely improve the expression level of the lock BBa_K1087004.